HOME >> Business >> R&D Support Business >> LabCyte EPI-MODEL

PRINT

R&D Support Business

LabCyte EPI-MODEL

LabCyte EPI-MODELThe LabCyte EPI-MODEL is produced by culturing human epidermal cells as a monolayer. After the human epidermal cells have proliferated, exposing their surface to the air causes it to keratinize*, creating a cultured epidermis model similar to the human epidermis.

*Keratinization: A process by which epidermal cells differentiate, losing their nuclei and intracellular organelles, as the interior of the cell is filled with aggregated keratin fibers. They become tough structures, both physically and chemically, that serve a barrier function against the external environment.

Characteristics of LabCyte EPI-MODEL

LabCyte structureLabCyte EPI-MODEL is a 3-dimensional reconstructed human epidermis model that is produced by culturing normal human epidermal cells to become multilayered. The structure of LabCyte EPI-MODEL is morphologically similar to that of human epidermis, consisting of the basal layer, spinous layer, granular layer and cornified layer.
LabCyte layers

Immunostaining

  • Cornified layer
    (filaggrin)
    filaggrin
  • Basal layer
    (laminin)
    laminin
  • Epidermal differentiation
    (transglutaminase)
    transglutaminase

Transmission Electron Microscopy

Basal layer
Basal layer
Spinous layer
Stratum spinosum
Granular layer
Granular layer
Cornified layer
Cornified layer

Cultivation Process

  • Day 1
    Day 1
  • Day 4
    Day 4
  • Day 7
    Day 7
  • Day 10
    Day 10
  • Day 14
    Day 14
  • Day 18
    Day 18
  • Day 21
    Day 21
  • Day 28
    Day 28

Pagetop

Products and Accessories

LabCyte EPI-MODEL12

  • LabCyte EPI-MODEL12 (1 plate)
    EPI-MODEL12
  • 12-well plate (1 plate)
    12-well plate
  • Assay medium 30ml (1 bottle)
    Assay medium

LabCyte EPI-MODEL24

  • LabCyte EPI-MODEL24 (1 plate)
    EPI-MODEL24
  • 24-well plate (1 plate)
    24-well plate
  • Assay medium 30ml (1 bottle)
    Assay medium

Skin irritation test set

  • LabCyte EPI-MODEL24 (1 plate)
    EPI-MODEL24
  • 24-well plate (4 plates)
    24-well plate
  • Assay medium 30ml (2 bottles)
    Assay medium

Accessories

  • Assay medium (100ml, 250ml)
  • MTT reagent
  • 12-well plate
  • 24-well plate
  • 96-well plate

Pagetop

Storage and Expiration Date

Store the unopened 3-dimensional cultured human epidermis model at room temperature. The expiration date is indicated on the label of the aluminum package. For better reproducibility of results, it is recommended to keep the storage period prior product use the same across experiments.

It is recommended that all culture inserts are used once the package is opened. Do not store this product in the provided assay medium, or in a CO2 incubator (37˚C, 5-10% CO2), as cellular metabolism is activated under these conditions.

The assay medium should be stored in a refrigerator (2-8˚C). The expiration date of the medium is indicated on the label of the bottle.

Pagetop

Method of Application

Basic procedure

  1. Application of test chemicals

    ApplicationRemovalRemoval

    Apply the test chemical to the surface of the tissue for the intended time period. ⇒ Remove the test chemical.

  2. Detection of viable cells

    RemovalRemovalMTT medium

    Remove the medium from the outside of the culture insert. ⇒ Fill each well with warm MTT medium and incubate for 3 hours.

  3. Measurement of optical density

    After the MTT reaction, remove the epidermal tissue from the culture insert.
    Make sure that tissues not treated by the test chemical are stained purple.
    PinchStained purple

    Transfer each tissue to a clean microtube.
    Add the extraction solvent to the microtube and incubate for more than 2 hours.
    Measure the optical density (OD) at 570 nm and 650nm.
    Extraction solvent

Skin irritation test using LabCyte EPI-MODEL24

Test procedure

S.O.P

Skin irritation test using the reconstructed human epidermal model LabCyte EPI-MODEL24

Result

Result from skin irritation test (54 chemicals)
Correlation

Other Applications

  1. Skin metabolic studies

    Experiments to investigate how substances are metabolized by skin enzymes, basic research on skin tissue, etc.

  2. Skin irritation test

    Experiments to investigate whether chemical substances cause skin irritation, experiments to assess the safety of drugs and cosmetics, etc.

  3. Percutaneous absorption test

    Experiments to investigate whether chemical substances, drugs or cosmetics can permeate the skin.

  4. Skin corrosion test

    Experiments to investigate the safety of chemical substances, studies on the safety of substance handling in chemical plants, etc.

Pagetop