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LabCyte EPI-MODEL

LabCyte EPI-MODELThe LabCyte EPI-MODEL is produced by culturing human epidermal cells on a culture plate. After the human epidermal cells have been cultured and proliferated, exposing their surface to the air causes it to keratinize*, creating a cultured epidermis model similar to the human epidermis.

*Keratinization: A process by which epidermal cells differentiate, losing their nuclei and intracellular organelles, and the interior of the cell is filled with aggregated keratin fibers. They become tough structures, both physically and chemically, that serve a barrier function against the external environment.

Characteristics of LabCyte EPI-MODEL

LabCyte structureLabCyte EPI-MODEL is a 3-dimensional reconstructed human epidermis model that is produced by culturing normal human epidermal cells to become multilayered. The structure of LabCyte EPI-MODEL is morphologically similar to that of human epidermis, consisting of the basal layer, spinous layer, granular layer and cornified layer.
LabCyte layers

Immunostaining

  • Cornified layer
    (filaggrin)
    filaggrin
  • Basal layer
    (laminin)
    laminin
  • Epidermal differentiation
    (transglutaminase)
    transglutaminase

Transmission Electron Microscopy

Basal layer
Basal layer
Spinous layer
Stratum spinosum
Granular layer
Granular layer
Cornified layer
Cornified layer

Cultivation Process

  • Day 1
    Day 1
  • Day 4
    Day 4
  • Day 7
    Day 7
  • Day 10
    Day 10
  • Day 14
    Day 14
  • Day 18
    Day 18
  • Day 21
    Day 21
  • Day 28
    Day 28

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Products and Accessories

LabCyte EPI-MODEL12

  • LabCyte EPI-MODEL12 (1 plate)
    EPI-MODEL12
  • 12-well plate (1 plate)
    12-well plate
  • Assay medium 30ml (1 bottle)
    Assay medium

LabCyte EPI-MODEL24

  • LabCyte EPI-MODEL24 (1 plate)
    EPI-MODEL24
  • 24-well plate (1 plate)
    24-well plate
  • Assay medium 30ml (1 bottle)
    Assay medium

Skin irritation test set

  • LabCyte EPI-MODEL24 (1 plate)
    EPI-MODEL24
  • 24-well plate (4 plate)
    24-well plate
  • Assay medium 30ml (2 bottle)
    Assay medium

Accessories

  • Assay medium (100ml, 250ml)
  • MTT reagent
  • 12-well plate
  • 24-well plate
  • 96-well plate

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Preservation and Expiration Date

Store 3-D cultured human epidermis model in the aluminum package at room temperature. The expiration date is indicated on the label of the aluminum package. It is recommended that the preservation period before its use should be invariable for better reproducibility in results.

If possible, use all the culture inserts after opening. It is not appropriate to store this product in the provided assay medium or the CO2 incubator (37˚C, 5-10% CO2), because the cellular metabolism is activated.

Please note that the assay medium should be stored in a refrigerator (2-8˚C). The expiration date of the medium is indicated on the label of the bottle.

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Method of Application

Basic procedure

  1. Application of test chemicals

    ApplicationRemovalRemoval

    Apply test chemical to the surface of the epidermis tissue for the intended period. ⇒ Remove the test chemical.

  2. Detection of viable cells

    RemovalRemovalMTT medium

    Remove the medium from the outer side of the culture insert. ⇒ Fill each well with the warmed MTT medium and then incubate for 3 hours.

  3. Measurement of optical density

    After the MTT reaction, pinch the epidermis tissue from the culture insert.
    Confirm that the tissues not treated by the test chemical are stained purple.
    PinchStained purple

    Put each tissue into a micro-tube .
    Add extraction solvent to the micro-tube and incubate for more than 2 hours.
    Measure the optical density (OD) at 570 nm and 650nm.
    Extraction solvent

Skin irritation test using LabCyte EPI-MODEL24

Test procedure

S.O.P

Skin irritation test using the reconstructed human epidermal model LabCyte EPI-MODEL24

Result

Result from skin irritation test (54 chemicals)
Correlation

Other Applications

  1. Skin metabolic studies

    Experiments to investigate how substances are metabolized by skin enzymes, basic research on skin tissue, etc.

  2. Skin irritation test

    Experiments to investigate whether chemical substances cause skin irritation, safety experiments on drugs, cosmetics, etc.

  3. Percutaneous absorption test

    Experiments to investigate whether chemical substances can permeate the skin, or to find out whether drugs or cosmetics infiltrate the skin.

  4. Skin corrosion test

    Experiments to investigate the safety of chemical substances, studies of the safety of substance handling in chemical plants, etc.

  5. Other types of research using skin

    In the future our lineup will also include cultured skin that includes the dermal layer, as well as skin models that incorporate melanocytes for use in research on melanin, which causes pigmentation spots and sunburn.

  6. Eye irritation test

    Experiments to investigate whether chemical substances cause eye irritation, safety experiments on drugs, cosmetics, etc.

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