LabCyte EPI-MODEL
LabCyte EPI MODEL is the 3D reconstructed human epidermis made by stratified culture of normal human epidermal keratinocytes. The tissue presents similar structures to that of normal human epidermal tissue with the basic epidermal structure (basal layer, spinous layer, granular layer, and stratum corneum). J-TEC has two types of tissue size, 24-well type (EPI-MODEL24) and 12-well type (EPI-MODEL12).
Listed in OECD Test Guidelines (TG439, TG431, TG442D)
Human reconstructed tissue models can be used to replace animal testing. LabCyte EPI-MODEL24 is listed in OECD test guidelines 439 (in vitro skin irritation test), 431 (in vitro skin corrosion test), and 442D (in vitro skin sensitization test).
EpiSensA - Skin Sensitization Test (OECD TG442D)
OECD test guideline 442D lists Epidermal Sensitization Assay (EpiSensA), an in vitro skin sensitization test developed by Kao Corporation utilizing EPI-MODEL24. EpiSensA quantifies the changes in the expression of marker genes associated with the activation of keratinocyte, which correspond to the key event 2 (KE-2) in the skin sensitization adverse outcome pathway.
EPI-MODEL 6D
EPI-MODEL 6D (6-day cultured product) is a model manufactured with the culture period shortened by one week, resulting in a thinner cornified layer compared to EPI-MODEL.
EPI-MODEL 6D exhibits more sensitive cytotoxicity due to its thinner cornified layer.
EPI-KIT
Prepare your own epidermal model by simply seeding and culturing keratinocytes in cell culture inserts.
